What will be the power value for SNP study in Sample size of 30?

What will be the power value for SNP study in Sample size of 30?

05 June 2018 7,037 0 View

How could i minimize the amplification of Allele specific primer?

when am doing ARMS PCR both WT and MT amplified equally? how to minimize WT amplification?

04 May 2018 8,795 3 View

How to calculate power value for Polymorphic studies?

how to calculate power value for Polymorphic studies and how to predict the sample size to get optimum power value ?

03 April 2018 3,391 0 View

How a SNP in intronic region of TCF7L2 lead to T2DM.?

How a mutation in introns affect the gene expression. if a SNP is not present in any downstream regulatory elements but in somewhere intermediate region of intron and how those polymorphism...

02 March 2018 5,925 3 View

Will the sequence specific primers for the real time PCR work with ordinary PCR amplification?

01 February 2015 710 5 View

I need ChemBioDraw ultra software in full version?

Can anyone suggest me how to download full version of ChemBioDraw for free

01 February 2015 1,341 0 View

Can anyone suggest how to avoid retention time shift in biogenic amine estimation using a UV-VIS detector?

08 September 2014 5,101 3 View

Can anyone suggest to me what the concentration or level of heavy metals are in human serum sample according to WHO guidelines ?

08 September 2014 3,746 8 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View