How could i minimize the amplification of Allele specific primer?

when am doing ARMS PCR both WT and MT amplified equally? how to minimize WT amplification?

04 May 2018 8,795 3 View

How to calculate power value for Polymorphic studies?

how to calculate power value for Polymorphic studies and how to predict the sample size to get optimum power value ?

03 April 2018 3,391 0 View

How a SNP in intronic region of TCF7L2 lead to T2DM.?

How a mutation in introns affect the gene expression. if a SNP is not present in any downstream regulatory elements but in somewhere intermediate region of intron and how those polymorphism...

02 March 2018 5,925 3 View

Will the sequence specific primers for the real time PCR work with ordinary PCR amplification?

01 February 2015 710 5 View

How to get the whole genome of the wistar albino rat?

I would like to design the primer pairs for Monoamine oxidase gene, can anyone suggest me how to get pre designed primers for monoamine oxidase or how to design it for the wistar albino rat from...

01 February 2015 3,663 0 View

I need ChemBioDraw ultra software in full version?

Can anyone suggest me how to download full version of ChemBioDraw for free

01 February 2015 1,341 0 View

Can anyone suggest how to avoid retention time shift in biogenic amine estimation using a UV-VIS detector?

08 September 2014 5,101 3 View

Can anyone suggest to me what the concentration or level of heavy metals are in human serum sample according to WHO guidelines ?

08 September 2014 3,746 8 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View