i am tying it on MS media with 2,4-D @ 2 mg/l; 0.5 mg/l kinatin, casein hydrolase. i have been trying it since 10 months. Cells are not dividing when transfer to suspension media. ---- Any suggestions?
Hi RK, you din't mentioned the cultivar. in general rice you suppose to get microcallus from scutellem region (if you use mature seed as a initial explant). would you be so kind to let me know the purpose of your study(i.e genetic t transformation or any other study) read the attached publications for further information. good luck
Thank you Krishna. I am using BPT variety to get friable callus. I want to do cell suspension cultures to test the elicitor activity of new compounds. Of-course i am using mature seed as explant. I am getting compact callus.