How to generate the P value or matrix value from aligned or multiple FASTA file?

More Darshan Rudakiya's questions See All

I want to make journal bearing test rig so which parameter consider for design?

if any one having test rig drawing please send me

14 February 2024 9,553 0 View

Is there any alternative method of preparative HPLC?

Hello All, I want to do chiral separation of organic Phosphate salt moiety. that can be done on Preparative HPLC, but in unavailability of preparative HPLC, is any alternative method to do so?

22 February 2023 8,507 0 View

EFFICACY OF PELVIC PNF TO IMPROVE TRUNK CONTROL, BALANCE AND GAIT PATTERN IN NEURO. Please fill up this Questionnaire by clicking on the link ?

Greetings! I, Darshan Prakashbhai Parmar, MPT student, from Government Physiotherapy College Jamnagar, am conducting a survey on 'EFFICACY OF PELVIC PNF TO IMPROVE TRUNK CONTROL, BALANCE AND GAIT...

13 July 2022 9,365 1 View

Are the sitting sequences added before the PCR primers arbitrary or specific to restriction endonucleases?

As I have started to learn primer designing, I have come across terms like restriction sites, sitting sequences, etc. I wanted to know if sitting sequences added before the restriction sites are...

25 November 2021 1,637 3 View

QCM have ability to tect in wave?

QCM have able to determined in wave / air genemoe or only microfluidic

27 April 2020 3,842 0 View

How to determine the concentration of silver nanoparticles using UV visible spectra?

I have synthesized silver nanoparticles using yeast want to determine its concentration using UV visible spectra? Can anyone help me out with it?

09 March 2020 9,231 3 View

Why TCR would inject even harmonics firing beyond 90 to 180 degree firing window.?

as we fire the TCR beyond the firing window of 90 to 180 degree current waveform gets positive/ negative bias. what is the reason behind that.?

20 June 2019 7,808 1 View

Where can I buy a pulse oximeter which stores raw photoplethysmography (PPG) data?

Hi, I need raw PPG signal for a project I am working on. I have tried searching for a pulse oximeter online but many do not allow access to the raw PPG signal. I am looking for a device which...

07 April 2019 1,097 8 View

How to avoid unwanted turn on of MOSFET due to ground bounce in full bridge AC-DC converter.?

At present i am working on 1 kW battery charger based on full bridge topology. the charger repeatedly failed due to shoot through. this is not due to overlapping of gate pulse as my maximum duty...

01 January 2019 7,208 3 View

Can anyone provide me the protocol for the leaf and root treatments of rice before subjecting it to ultramicrotomy for the grid preparation of TEM?

I am looking forward to learn the protocol to treat the leaf,stem and root before embedding them in resin and subjecting the resin bead to ultra-microtomy sectioning prior to the grid preparation...

29 July 2018 1,311 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to do Mann-Whitney U test with Bonferroni corrected p-values?

Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...

31 July 2024 6,464 5 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Temim Deli

Dear Darshan,

I suggest using the package SeqFeatR. The software provides user friendly methods for the identification of sequence patterns that are statistically significantly associated with a property of the sequence. Please find attached the user guide manual for help.

Good luck!

Darshan Rudakiya

Thank you sir for your help