Can anyone provide me the protocol for the leaf and root treatments of rice before subjecting it to ultramicrotomy for the grid preparation of TEM?

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Vladimir Dusevich

Since you are not getting a specific protocol, may be those will be of some help:

http://www.ou.edu/research/electron/bmz5364/fix-bot.html

https://www.huck.psu.edu/content/instrumentation-facilities/microscopy-and-cytometry-facility/faq/other/sample-preparation

Marpha Telepova-Texier

Next one

Robards AW, Sleytr UB. 1985 Low temperature methods in biological electron microscopy. [Practical methods in electron microscopy (ed. Amsterdam.: Glauert, A.M.) 10.] Elsevier 551 pp.. Review by E.A.Munn in: J. Microsc. 156 (1989) 263-5. Water and its properties; freezing and thawing; X-ray microanalysis of frozen specimens; thin sectioning of frozen specimens; freeze-drying; freeze-fracture replicas; autoradiography; freeze-substitution and low-temperature embedding. textbk, Technique Microscopy Staining, Ultrastructure: TEM, Review article General article (📷, 185810146).