Hello, linearity important to have more reliable answer.

linearity of sandwich assay curve for protein sample detection is called linear regression.

Example for calculation by non-linear curve is linked below and attached.

https://www.google.com/amp/blog.minitab.com/blog/adventures-in-statistics-2/curve-fitting-with-linear-and-nonlinear-regression%3fhs_amp=true

Hope this info will help you.

Good luck!

Hello, linearity important to have more reliable answer.

linearity of sandwich assay curve for protein sample detection is called linear regression.

Example for calculation by non-linear curve is linked below and attached.

https://www.google.com/amp/blog.minitab.com/blog/adventures-in-statistics-2/curve-fitting-with-linear-and-nonlinear-regression%3fhs_amp=true

Hope this info will help you.

Good luck!

Please see this link, it can be done by prism software.

https://s3.amazonaws.com/cdn.graphpad.com/faq/1757/file/P4-Nonlinear%20standard%20curves.pdf

It would be reliable to analyze your sample within linear range. Dilute or concentrate your sample to make sure the readout within linear range is important. .