How to find the degree of substitution in N-succinyl chitosan?

More Nirmla devi's questions See All

How indoor air pollution is sampled using TSI quest EVM air monitoring instrument?

please suggest the research paper related to the instrument is used in it.

16 July 2024 8,453 1 View

I have a doubt in finding the amount of ferromagnetic and antiferromagnetic contribution in a canted system?

Is it possible to differentiate or calculate, the amount of ferromagnetic and antiferromagnetic contribution in a canted system? (Weakly frustrated canted antiferromagnetic system)

25 June 2024 6,210 0 View

I have a doubt in Rietveld refinement error bars? how to find out in GSAS II software?

In many papers, they have given the atomic position, lattice parameters, bond length, bond angle etc. values with (). what is the value of inside the bracket? If it is error bar how to find in...

13 June 2024 7,607 2 View

Accepted (Systematic Literature Review)...?

Menurut Bapak/Ibu, apakah editor jurnal lebih memilih Systematic Literature Review 10-20 tahun daripada yang menelaah 5 tahun? In your opinion (Mr and Ms), what do you think, journal editors...

26 May 2024 140 2 View

Under what circumstances, a researcher can use quotations with names of respondents tagged to them, in a qualitative research study?

I am preparing a case study of a social work professional organisation which is non-existent now but had a glorious past. For this, I interviewed around 20 members of the association to understand...

18 May 2024 6,656 3 View

AI tools in Software Testing Automation?

Machine Learning or AI Tools roleplay in the Automation of Software Testing

17 February 2024 159 0 View

Why passage number 5?

In order to prepare conditioned media from primary cultured dental pulp cells, passage 5 is widely reported. May I have some scientific explanation despite of the inadequate quantity of the cell....

09 January 2024 898 2 View

Are nitrates being toxic to aquatic microrganisms? If yes elaborate on genotoxicological effects and molecular mechanisms?

Any harmful effects on the genetic material of microbes due to nitrate exposure. When nitrate is uptake by the bacterium, molecular mechanism involved how it is causing DNA or RNA damage?

03 December 2023 9,886 2 View

What does a layer formation indicates during NK cell cultre?

I noticed layer was formed during NK cell culture (initial day) of a cancer patient. Could it be platelet aggregation? What are the causes of layer formation in NK cell culture?

23 November 2023 6,674 0 View

How should I prepare GC-MS sample of algal biomass?

Please share the GC-MS sample preparation of algal biomass. My research is focused and interested in lipid content should I go for whole biomass or extract lipid and then perform the FAME analysis...

14 November 2023 3,946 2 View

Does anybody know why my chitosan/pva/gelatin hydrogel dissolve in PBS?

My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...

28 July 2024 2,794 6 View

How i figuer out the weight of curcumin powder?

I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?

10 July 2024 2,462 1 View

What are the strategies to improve the stability of chitosan nanoparticles in DMEM (cell culture media)?

Hi, I am synthesising chitosan nanoparticles using the ionic gelation method and I am characterising the nanoparticles in distilled water and in DMEM (cell culture), however the zeta potential in...

01 July 2024 1,526 1 View

How to load Remedesivir drug into mesoporous nanoparticles?

Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...

01 July 2024 889 3 View

Any better protocol for entrapment efficiency of curcumin?

I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...

01 July 2024 7,007 1 View

Can I magnetic stirrer chitosan 24 hours hours but 6 hours stirring 10 hours break like that?

I need to magnetic stirrer chitosan for 24 hours. In this case, if I magnetic stirrer the chitosan solution for 6 hours, then I take 10 hours of breaks. I followed this 4 times so that the overall...

31 May 2024 9,202 0 View

How to solubilize Chitosan( high molecular weight) in a aqueous solution ?

Hi everyone, there is my issue: I try to synthetize nanoparticule of Chitosan according with this study doi: 10.1016/j.colsurfb.2011.09.042 I try it with HMW Chitosan (340-360kDa) but when I put...

29 May 2024 1,763 2 View

Problem while making hydrogel from chitosan and gelatin ?

A uniform mixture of chitosan and gelatin is made by dissolving in acetic acid, after that the mixture is transferred to a plastic petri plate kept at -20 C for 24h and then lyophilized. But i am...

29 May 2024 7,367 4 View

What method is good for doing in vitro degradation study of PCL microsphere by change in weight calculation or change in diameter (from microscope)?

I have prepared some polymeric microspheres from chitosan and PCL. However while performing in vitro degradation study, I found difficulties in both above mentioned methods. Here I am mentioning...

20 May 2024 6,114 4 View

Silver chitosan nanoparticles were synthesized using cold plasma, we observed that the solution reverted to being transparent after one day. Reasons?

We prepared a solution using silver nitrate, chitosan, and sodium tripolyphosphate to synthesize silver chitosan nanoparticles. This solution was subjected to cold atmospheric pressure plasma for...

19 May 2024 9,317 2 View

Alaa Kareem Niamah

Hi,

You can use FTIR.

Article Synthesis and Characterization of Solid Polymer Electrolyte ...

Alaa Jabbar Abd Al-Manhel

Hi Devi

I agree with Alaa Kareem Niamah ..you can use FTIR and NMR.

Regard

Nirmla devi

Thank you so much @ A.K.Niamah @ A.J. Al-Manhel @E.B. De Oliveira

Avinash Kumar

Use NMR to get the degree of substitution.

for details refer my paper

Article Stability and acidic pH-mediated leakage of guest molecules ...

Thank you@Avinash Kumar

Arvind Singh Chandel

Remaining NH2 can be estimated by TNBS assay

Abdelkader BOUAZIZ

Dear all, please check the following links and attached documents. My Regards

https://doi.org/10.1016/j.ijbiomac.2012.07.029

https://www.researchgate.net/publication/314092234_Synthesis_of_N-Succinyl-_and_N-Glutaryl-Chitosan_Derivatives_and_Their_Antioxidant_Antiplatelet_and_Anticoagulant_Activity

Thank you so much@ Abdelkader BOUAZIZ @ A.S. Chandel