How can we determine persistence criteria (run length and frequency) for Typical Meteorological Year (TMY) creation in Sandia Method ?

While trying to select typical months to generate a typical meteorological year (TMY) for weather files used in building performance simulation, 5 candidate months are determined at first step...

15 November 2019 8,748 2 View

Anybody have idea about incorrect Tm values from High Resolution Melting Analaysis that SSR markers has been used ?

In my HRM analyzes, although I use the same samples in the same analysis, I get different Tm values. And I've repeated it many times, but there has always been a discrepancy. For example, even...

15 May 2019 4,771 10 View

Why I taking too low DNA amount from the wild type Einkron wheat?

Despite I repeat it three times to get DNA from the Einkorn. I couldn't take high amount from it. Could it be related to its cell structure? or I doing some mistake? Note: The protocol had been...

07 April 2018 7,482 6 View

Dispersion of pDNA loaded nanoparticles?

Dear all, I encapsulate pDNA into my nanoparticles, however, my particles are extremely sensitive to pH changes. For radical pH changes like from 4 to 7.4, they aggregate quickly. It cannot be...

21 November 2017 6,862 2 View

What do you think about the future of summability and its applications?

At the end of the nineteenth century, many researchers concentrated on various alternative methods based on the theory of infinite series. These methods have been combined under a single heading...

26 February 2017 1,766 11 View

How does a linear polymer dissolve in a solution and What do I see in DLS?

Hi everyone, I have deacetalyzed low molecular weight chitosan polymer which is soluble in %0.5 (v/v) acetic acid solution. I prepared chitosan/acetic acid solution (0.5 mg/mL) and used DLS to see...

17 October 2016 668 11 View

What is the main common key features of spree killers?

03 April 2015 2,407 1 View

What is the actual effects of global warming on total ice mass in antarctica?

"[Ice] is expanding in much of Antarctica, contrary to the widespread public belief that global warming is melting the continental ice cap." (Greg Roberts, The Australian) This sentence may be...

23 March 2015 5,707 7 View

Does anybody have any experience in Bacillus subtilis Secretory Protein Expression System from Takara?

We have been struggling to get positive transformants when we used the commercial kit from TAKARA, Cat No: 3380, the included B. subtilis host strain is RIK1285. We have been following their...

22 March 2015 8,737 7 View

What is the relationship or intersection point between Social Ecology and Social Psychology in terms of sociological studies?

Even though these two notions are conceptually different from each other, these are get involved in urban sociology and also related to urban ecology. And the intersection of these two concepts...

31 December 2014 4,869 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View