Any electrolyte has it's own ionicity - either it's cationic or anionic, also sometimes non-ionic. So how one can find out its ionic charge?
I don't really understand this question. In solution, both cations and anions from any electrolyte are present. Whether the electrolyte is a strong or weak electrolyte in solution will depend upon its dissolution constant, among other factors.
Hi My molecule is displaying hybrid quenching (both static and dynamic). I would require help to plot Fo/F(eV[Q]) -1 Vs [Q], how to calculate the value of 'V'?
14 May 2023 5,527 0 View
I want to calculate the volume of Voronoi Polyhedra so that I can calculate effective free volume. I have no idea how to calculate the volume of Voronoi Polyhedra. I have tried using ovito but I...
28 May 2022 9,905 0 View
I am trying to perform LULC prediction modelling on LULC maps prepared using LANDSAT 5 TM and LANDSAT 8 OLI images, in the Land Change Modeler in IDRISI Selva. I first converted Classified image...
18 August 2021 1,102 2 View
I have been able to reactivate EBV in different EBV+ cell lines in vitro. I confirm the reactivation by measuring the expression of lytic genes such as BZLF1 at protein and RNA level by Flow...
20 October 2020 8,584 0 View
We want to run a mixed effects model for our experimental design using lme4 package in R and want to confirm if our model is specified correctly. Our design involves two random factors...
17 April 2020 6,473 1 View
14 April 2020 4,013 0 View
For calculating entrapment efficiency using UV-Vis, the unencapsulated drug is discarded with supernatant so that you measure unencapsulated drug in supernatant via UV-vis absorption.However, the...
13 February 2020 9,351 1 View
I have used Landsat OLI and TM images for 2 different time periods and classified them using supervised classification.
26 August 2019 3,791 4 View
What happens if we accidentally add more (5 ml of 200 mM) sodium pyruvate to 500 ml DMEM cell culture media that already contains 110 mg/ml sodium pyruvate in it (from company)? How will it...
29 November 2017 6,709 2 View
There are hormonal replacement options but why not drugs to bypass the coagulation cascade altogether?
04 October 2017 7,267 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View