How to test interactions in mixed-effects model?

We want to run a mixed effects model for our experimental design using lme4 package in R and want to confirm if our model is specified correctly. Our design involves two random factors...

17 April 2020 6,281 1 View

Regarding entrapment efficiency calculation using UV-Vis?

For calculating entrapment efficiency using UV-Vis, the unencapsulated drug is discarded with supernatant so that you measure unencapsulated drug in supernatant via UV-vis absorption.However, the...

13 February 2020 9,181 1 View

How to create post-classification change matrix and a change map there of?

I have used Landsat OLI and TM images for 2 different time periods and classified them using supervised classification.

26 August 2019 3,651 4 View

What if Sodium pyruvate is added in excess in DMEM cell culture media?

What happens if we accidentally add more (5 ml of 200 mM) sodium pyruvate to 500 ml DMEM cell culture media that already contains 110 mg/ml sodium pyruvate in it (from company)? How will it...

29 November 2017 6,582 2 View

What tests can be done during fungal fermentation to keep a check?

While doing fermentation of any gungal strain, what other check points can be kept for example growth curve, reducing sugar, growth density.,etc.

21 October 2012 2,131 1 View

How can dextranase activity in sugarcane juice be assessed?

As the presence of dextran is very low in sugarcane, how one can judge that the dextranase has degraded the dextran present in the sugarcane juice? How quickly can we find out whether the enzyme...

30 May 2012 4,693 1 View

Why wasn't the survey interview questions published alongside the paper-Agenda setting function of mass media published in summer 1972?

01 January 1970 2,128 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View