Has anyone successfully reactivated EBV in Farage cells?

Srishti Chakravorty @Srishti-Chakravorty

21 October 2020 0 9K Report

I have been able to reactivate EBV in different EBV+ cell lines in vitro. I confirm the reactivation by measuring the expression of lytic genes such as BZLF1 at protein and RNA level by Flow cytometry and qPCR respectively. However, I am unable to reactivate EBV in Farage cells, a Diffuse Large B cell Lymphoma (DLBCL) cell line. I have tried a number of different methods to induce reactivation but nothing has worked well so far. Does anyone have an experience with these cells or have any suggestions? Thank you.

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