How to extract total bacterial RNA from Salmonella infected Macrophages?

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How to download a spatial subset of the new worldclim 2.1 layers using the R console?

Hello I have tried to download the new version of the worldclim bioclimatic layers in the R console, since this way I can download spatial subsets. Does anyone have a code that works with the new...

10 September 2020 1,948 5 View

How to derive the expression for the View Factor of a configuration of two bodies, starting from the general case ?

Hi all : Please, I need some help on Heat Radiation Transfer: How I can derive the formula of the View Factor for a particular configuration, in a way of ending with an expression for the VF as...

15 May 2020 4,929 6 View

Radiation Heat Transfer between a Hot body and a real Surface ?

Hi all , How can I consider the Optical properties of a surface into the Stefan-Boltzmann law for a problem of Radiation Heat Transfer between a hot Body and a real Surface (namely, a surface...

15 May 2020 9,449 8 View

Is this a well defined Integral ? If so, does anyone can help me to solve it ?

Dear all : I need to solve the following integral (attached as an image file) The context is on the calculation of View Factors in Radiation Heat Transfer I worked out the this expression from...

15 May 2020 7,500 16 View

How to calculate the Solar Radiative Flux arriving to a Solar Plate Collector placed on the surface of Earth ?

Hi all, I'm trying to perform a calculation on the Flux due only to radiation, received at the surface of a Flat Plate Solar Collector placed on the surface of the Earth. The collector is tilted...

14 May 2020 604 24 View

Air Velocities and Temperature Distributions inside an enclosed air cavity, modeled in COMSOL Multiphysics ?

Dear all : I'm trying to solve the following problem using COMSOL Multyphysics. I have an enclosed geometry (please see the attached images) with a top flat surface (the celing) and an undulated...

19 March 2020 7,455 5 View

Simulation of an Air Solar Plate Collector in COMSOL Multiphysics. Problems with defining the Boundary Conditions ?

Dear all: I need help, I hope there is someone out there who can help me. I finish drawing the 2D geometry of an Air Solar Collector with an undulated absorber plate (in contrast with a flat...

19 March 2020 5,935 9 View

Does the value for the Seebeck Coefficient (S) measured with a Voltmeter can really be taken as the S of the test material ?

Hello to all : This question is originated from the idea that every material (except for Superconductors) has a Seebeck Coefficient (S) different than zero, and from the idea that in every case...

19 March 2020 4,192 5 View

Diffusion of Carbon atoms in Bismuth Telluride ?

Dear Colleagues : Does anyone have literature referencing the diffusion process of Carbon (I mean Carbon atoms) into Bismuth Telluride (Bi2Te3) or into some other compound alike ? E.g. PbTe,...

05 March 2020 2,976 2 View

Chemical Stability of the System Bi2Te3 with Graphene ?

Hello to all: I'm trying to think in the following interesting problem: I have a system which are polycrystals (grains) of Bi2Te3 (actually this compound is a quaternary alloy of Bi, Te, Se and...

05 March 2020 4,832 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Awatif Hameed Issa

Dr. Dear

A highly multiplexed and sensitive RNA-seq protocol for simultaneous analysis of host and pathogen transcriptomes

Roi Avraham, et.al. 2016

Nature Protocols11,1477–1491(2016)doi:10.1038/nprot.2016.090Published online21 July 2016. abstract attached , suggested to you.

Good luck.

Ting Li

Hi, this paper may be useful.

Dual RNA-seq of pathogen and host

Alexander J. Westermann1, Stanislaw A. Gorski1 & Jörg Vogel

Nature reviews. Microbiology, 09/2012, Volume 10, Issue 9

Freddy Franklin

Thank you Ting Li and Awatif Hameed Issa. Really appreciate the help.