30 May 2023 1 5K Report

I am doing TLC on moringa saponin but where can I get a standard moringa saponin?

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What is the role of culture in training and development, and how can multinational organisations design training programs?

How does culture influence the specific learning needs and preferences of participants in training and development programs? How do cultural factors influence participant engagement and motivation...

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Can you know the spot of the earth depth profile base on my data??

If you know how to look the earth depth profile you will know how to look the earth lat-long profile

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What are some of the simple yet effective methods of extracting and purifying Saponin from plants??

Working on Moringa saponin isolation and characterization. I wonder how does moringa saponin differ (structurally) from Quil A and its derivative, QS-21?

23 May 2023 2,656 1 View

How to download a spatial subset of the new worldclim 2.1 layers using the R console?

Hello I have tried to download the new version of the worldclim bioclimatic layers in the R console, since this way I can download spatial subsets. Does anyone have a code that works with the new...

10 September 2020 2,192 5 View

What is the best way to humidify H2 gas to 80% absolute humidity?

I want to feed an electrolysis cell with a 80:20 H2O:H2 gas mixture at a rate of 100 ml/min. What is my best chance of getting a stable gas mixture at low flow rates?

08 April 2020 1,332 4 View

¿is Vasa protein only specific to germ cells?

Currently, I've been working with inmunofluoresce for my thesis, and the aim of my project is to detect the germ cells, in the early stages of embryonic development for an insect. Before doing...

22 October 2018 4,099 2 View

How to obtain the slope for a curve (Absorbance against time) for Initial velocity from the polynomial equation?

Good Day. I'm working on enzyme kinetics and I require to plot Lineweave -burk/Eadie-Hofstee graphs/Hanes as my final results. I have plotted a graph (absorbance against time ) for five substrate...

07 May 2018 287 4 View

Any suggestions for microscopic traffic simulator that is capable of modelling vehicle path in 2-dimensions (not as discreet lane keep or jump)?

I find lane-based microscopic simulation models inadequate for modelling realistic trajectories of human driven vehicles and highly automated vehicles that follow a 2D path. Any suggestions or...

12 July 2017 2,819 7 View

Have anyone bibliography about image and social memory?

I need bibliography about relation between image and social memory. If someone has something I thank you.

03 May 2017 1,050 0 View

Missing bands in the positive control (pool DNA)?

Good day everyone. I've carried out mPCR for the confirmation of Non Typhoidal Salmonella using DNA extracted kit( G-spin from INTRON ) .While all the 5 strains (S. Typhimurium, Enteritidis,...

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Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

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GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

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Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

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How can I measure the thickness of thin film by Uv-Vis?

How can I measure the thickness of thin film by Uv-Vis?

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Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of  absorption chromatography?

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Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

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For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

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How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

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