Freddy Franklin

3 Questions 4 Answers 0 Followers

Questions related from Freddy Franklin

How to obtain the slope for a curve (Absorbance against time) for Initial velocity from the polynomial equation?

Good Day. I'm working on enzyme kinetics and I require to plot Lineweave -burk/Eadie-Hofstee graphs/Hanes as my final results. I have plotted a graph (absorbance against time ) for five substrate...

08 May 2018 270 4 View

Missing bands in the positive control (pool DNA)?

Good day everyone. I've carried out mPCR for the confirmation of Non Typhoidal Salmonella using DNA extracted kit( G-spin from INTRON ) .While all the 5 strains (S. Typhimurium, Enteritidis,...

09 September 2016 3,152 3 View

How to extract total bacterial RNA from Salmonella infected Macrophages?

Hi everyone. I'm about to start my Masters project and I'm looking for efficient methods or even kits to extract total bacterial RNA from Salmonella infected macrophage for transcriptome...

14 August 2016 834 3 View