I would like to perform an untargeted LC-MS analysis of the cell-free supernatant of bacterial culture to analyse all the possible metabolites present in the cell-free supernatant. Here I am attaching the protocol that I will follow to send the sample. Can I send the sample following this protocol?
Seems ok for non-targeted secretome analysis...Alternatively, you may also perform fractionation using tertiary solvent extraction or you may request to be applied to increase annotation coverage...But, It is still sufficient for LC-based discovery analysis...
To extract a sample for LC-MS (liquid chromatography-mass spectrometry) analysis, follow these general steps:
1. Sample Preparation: Begin by preparing your sample according to the specific requirements of your analysis. This may involve homogenizing or diluting the sample, depending on its nature.
2. Extraction: Choose an appropriate extraction method based on the properties of your sample. Common extraction methods include liquid-liquid extraction, solid-phase extraction, and protein precipitation.
3. Centrifugation: If necessary, centrifuge the extracted sample to separate the supernatant from any solid particles or debris.
4. Filtration: Use a syringe filter or centrifugal filter to remove any particulates or impurities from the extracted sample.
5. Transfer: Transfer the filtered sample into a vial suitable for LC-MS analysis.
6. Storage: Store the prepared sample in appropriate conditions (e.g., refrigerated or frozen) until it is ready for analysis.
It's important to note that these steps are general guidelines and may need to be adjusted based on the specific requirements of your LC-MS analysis and the nature of your sample. Always refer to the standard operating procedures and guidelines provided by your laboratory or analytical instrument manufacturer for best results.
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