Sanhita Saha

7 Questions 4 Answers 0 Followers

Questions related from Sanhita Saha

Solvent to dissolve bacterial metabolite?

I have extracted metabolite from a bacterial cell. I have to check the antimicrobial activity of metabolite. which solvent can I use to dissolve that metabolite as it contains both polar and...

06 June 2023 2,985 3 View

I would like to know if the functionality of the buffer gets degraded after autoclaving it?

I need to prepare PBS buffer. I would like to know if the functionality of the buffer gets degraded after autoclaving it?

09 March 2023 8,664 1 View

How to do sterilization of MRS broth containing 0.4% phenol?

I have to prepare MRS broth and add 0.4% phenol to that media . How can I sterilize this media containing phenol?

05 September 2022 6,940 1 View

What does the "significant differences between treatments" mean?

I have attached a picture name as Image-1 from the research paper Li et al. 2021. here in Fig-3 this is written that " The different letters (a-d) indicate significant differences between...

17 April 2022 5,767 3 View

Do I need to separate metabolites into polar and non-polar category before doing the C18 LC column or HILIC, and if so, then how can I separate that?

I have a bacterial metabolite. I want to identify both polar and non-polar metabolites present in the sample. Do I need to separate metabolites into polar and non-polar categories run separately...

07 March 2022 7,080 5 View

I have lysed the bacterial cell with methanol and take the supernatant after centrifugation.How do I isolate metabolite from supernatant?

I want to study the LC-MS analysis of a bacterial metabolite.The bacteria is a carotenoid producing bacteria. I have added methanol to the cell pellet and then undergo sonication for cell...

27 February 2022 3,122 4 View

I have sonicated a gram positive bacterial cell at 40% amplitude with 30 sec on/off for 20 minutes. But the cells are not lysing. what should I do?

I want to perform an untargeted lc-ms analysis of bacterial metabolite. Generally, we break down the cell of this bacteria by dissolving the pellet with methanol and then give heat treatment for...

20 February 2022 2,900 3 View