Sanhita Saha

13 Questions 4 Answers 0 Followers

Questions related from Sanhita Saha

Is it good to have antibiotic resistance property for probiotic?

To prevent intestinal disorders, probiotics can be rendered multidrug-resistant to survive in the presence of co-administered antibiotics, and they may also transmit the antibiotic-resistance gene...

02 April 2024 8,020 2 View

How to extract sample for LC-MS analysis?

I would like to perform an untargeted LC-MS analysis of the cell-free supernatant of bacterial culture to analyse all the possible metabolites present in the cell-free supernatant. Here I am...

05 November 2023 6,119 2 View

Is there any possibility of degradation of any compound present in the sample with the following drying processes?

I have extracellular as well as intracellular metabolite. I have dried the sample both by lyophilizing and air drying at room temperature. It usually takes one week to get the powdered sample...

03 November 2023 9,405 1 View

Up to what percentage DMSO can be used so that it does not have any toxic effect?

Up to what percentage DMSO can be used so that it does not have any toxic effect

12 October 2023 9,845 1 View

How to separate hydrophilic compound and hydrophobic compound?

I have an intracellular metabolite which contains both hydrophilic and hydrophobic compounds. What is the procedure for separating hydrophilic compounds and hydrophobic compounds?

12 October 2023 7,482 3 View

Food grade solvent to dissolve metabolite consist of both hydrophilic and hydrophobic compound?

I have extracted metabolite from probiotic bacteria and lyophilized the sample. I have to check the cytotoxicity of that metabolite. Both hydrophilic and hydrophobic compounds are present in the...

05 October 2023 4,038 2 View

Solvent to dissolve bacterial metabolite?

I have extracted metabolite from a bacterial cell. I have to check the antimicrobial activity of metabolite. which solvent can I use to dissolve that metabolite as it contains both polar and...

06 June 2023 2,992 3 View

I would like to know if the functionality of the buffer gets degraded after autoclaving it?

I need to prepare PBS buffer. I would like to know if the functionality of the buffer gets degraded after autoclaving it?

09 March 2023 8,670 1 View

How to do sterilization of MRS broth containing 0.4% phenol?

I have to prepare MRS broth and add 0.4% phenol to that media . How can I sterilize this media containing phenol?

05 September 2022 6,946 1 View

What does the "significant differences between treatments" mean?

I have attached a picture name as Image-1 from the research paper Li et al. 2021. here in Fig-3 this is written that " The different letters (a-d) indicate significant differences between...

17 April 2022 5,773 3 View

Do I need to separate metabolites into polar and non-polar category before doing the C18 LC column or HILIC, and if so, then how can I separate that?

I have a bacterial metabolite. I want to identify both polar and non-polar metabolites present in the sample. Do I need to separate metabolites into polar and non-polar categories run separately...

07 March 2022 7,084 5 View

I have lysed the bacterial cell with methanol and take the supernatant after centrifugation.How do I isolate metabolite from supernatant?

I want to study the LC-MS analysis of a bacterial metabolite.The bacteria is a carotenoid producing bacteria. I have added methanol to the cell pellet and then undergo sonication for cell...

27 February 2022 3,128 4 View

I have sonicated a gram positive bacterial cell at 40% amplitude with 30 sec on/off for 20 minutes. But the cells are not lysing. what should I do?

I want to perform an untargeted lc-ms analysis of bacterial metabolite. Generally, we break down the cell of this bacteria by dissolving the pellet with methanol and then give heat treatment for...

20 February 2022 2,905 3 View