I am working on miRNA extraction from FFPE samples. I am trying to improve the yield and quality of the extract. Afterward, qRT-PCR analysis is desired.
I would be grateful if someone shares how to cope with this problem.
Recently, I conduct xylene deparaffinization, proteinase K digestion. Then, I used TRIzol/TRIreagent for the extraction and also glycogen co-precipitation. But unfortunately, the yield is poor.
We have used Qiagen's miRNeasy FFPE Kit with good results, at least for tumor samples.
Very small RNA or even DNA fragments like miRNAs generally are a bother to isolate.
We also use the Qiagen product. I am not sure the kit or any kit can do much to "improve" the quality of the RNA but the quantity is generally fine. The quality depends more on how well the tissue was fixed and embedded and generally is pretty bad when using standard routine pathology material.
MiRNEasy kit works very well with high-yield. There used to be a very useful kit for miRNA check from SABiosciences which was bought out by another company. But I highly recommend going with that.
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- Biological Science
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- Nucleic Acids
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- microRNA