How to extract cholesterol from tissues? Manually how we can do that in lab without buying any kit?

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Jai Ghosh

Basically the procedure is same. Only thing in tissue extraction, you need to homogenize the tissue taking care to see that the cholesterol is not oxidized. Then simply extract it with acetone and purify it by crystallization. However I am giving you a reference which you may refer in case you need it.

E.T.Martucci∗S.V.Borges ,

Journal of Food Engineering

Volume 32, Issue 4, June 1997, Pages 365-373

Vijaya Ragavan

Thank you sir.@ Jai Ghosh