How to exprice protein in supurnatent by avaiding pellet expristion

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Where to start the Analytical model for Dry sliding wear of metal surface ?

I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.

29 July 2024 6,115 0 View

While doing Chronoamperometry, when the light is turned off, why the current is not decreasing sharply?

Here, I have used Ag/AgCl as reference electrode and 0.5M KOH.

29 July 2024 4,538 3 View

Is it possible to dock more than one drug in the same active site or pocket of a protein to study synergistic/antagonistic/additive properties?

I wanted to know whether we can observe the synergistic/antagonistic/additive properties of combinations or mixtures of compounds through docking analysis. But during docking preparation any...

28 July 2024 7,413 6 View

Validated HPLC method for the estimation of the Amphotericin B ?

During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...

08 July 2024 1,330 0 View

Regarding green consumer behaviour ?

HOW TO WRITE GREEN CONSUMER BEHAVIOUR AS A LITERATURE REVIEW

28 June 2024 2,661 0 View

Can the atomic % and weight% of a doping element decrease with increasing its concentration in the resulting compound? If yes, then how?

I found atomic% and weight % of doping element decreased in the resulting compound on increasing its concentration.

28 May 2024 2,455 2 View

How do I perform a dose response curve and get IC 50 values for herbal extract powder?

I need to find out the desired dose for my poly herbal formulation. Please guide me how to get the figures

12 May 2024 8,290 1 View

CNN model and Multi-output regression?

Dear All, I have an imagery with a single fish species within each image along with a list of morphometric measurements of the fish (length, width, length of tail, etc). I would like to train a...

28 April 2024 2,090 2 View

How to calculate the stress of material using XRD data?

Is there any formula to calculate stress

20 April 2024 8,612 2 View

Any studies/PhD thesis that has taken pan country as the sample and used FGD for studying a public health problem?

The pan-country study could have further divisions/categorization based on different criteria. Any recommendations would be greatly appreciated. Thank you!

27 March 2024 991 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View