How to exprice protein in supurnatent by avaiding pellet expristion

i regulaarly grow in 28 degers in wild type but i mutate alanine to cystine if it grow in 28 it goes to pellet 0.5mM IPTG 5h after that i cheaked 18 degres 0.1mM IPTG 12h the yield little bit...

21 July 2017 5,187 2 View