What is the equation of detection n= of DNA copies in conventional PCR product?

IS there is equation for detection of n= of DNA copies in conventional PCR product by measuring pcr product against spectrophotometer

31 December 2017 7,720 3 View

Can i perform 2 steps real time pcr run on one step?

to perform RT PCR to RNA sample the used technique 1st convert RNA to DNA in separate reaction then perform replication can i make them in one step?

11 December 2017 4,516 3 View

Can real time pcr work as conventional pcr?

we have a real time pcr applied biosystems can we use it as thermocycler only or cant ?

05 June 2017 9,749 3 View

I used arginine hydrolysis broth but didn t give any positive result can anyone explain this?

08 September 2016 6,526 4 View

No title

10 November 2015 9,475 3 View

What are scientific journals for publish with impact factor between 2.5 to 3 and its cost 50 to 100 $?

scientific journals for publish with impact factor between 2.5 to 3 and its cost 50 to 100 $

08 September 2015 8,565 0 View

Is anyone working on an emeA efflux pump in enterococci?

Can it work on whole DNA or plasmid only? What is its condition to work on PCR ?

10 November 2014 9,215 2 View

How can I convert PM into ul ?

PM=?ul

03 April 2014 3,095 2 View

How can I dilute the master mix for use?

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03 April 2014 1,108 2 View

How can i prepare master mix for a PCR reaction?

How can i prepare master mix for a PCR reaction?

03 April 2014 8,292 21 View

No title

02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

No title

28 February 2021 9,936 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View