How can I extract DNA by Tris–EDTA buffer and Triton X 100?

More Aalaa Samir's questions See All

What is the equation of detection n= of DNA copies in conventional PCR product?

IS there is equation for detection of n= of DNA copies in conventional PCR product by measuring pcr product against spectrophotometer

31 December 2017 7,838 3 View

Can i perform 2 steps real time pcr run on one step?

to perform RT PCR to RNA sample the used technique 1st convert RNA to DNA in separate reaction then perform replication can i make them in one step?

11 December 2017 4,630 3 View

Can real time pcr work as conventional pcr?

we have a real time pcr applied biosystems can we use it as thermocycler only or cant ?

05 June 2017 9,871 3 View

How to examine lecithinase activity of Enterococcus spp. on egg yolk agar ?

i prepared egg yolk agar 3%, 1% egg yolk (v/v) on BH agar but cant have result how can prepare it?

04 May 2017 6,647 4 View

I used arginine hydrolysis broth but didn t give any positive result can anyone explain this?

I used this broth according to manufacture recommendation in preparing the media http://www.titanmedia.in/images/upload/td/TM%201140_ARGININEDIHYDROLASEBROTHTM-1140.pdf

08 September 2016 6,682 4 View

What are scientific journals for publish with impact factor between 2.5 to 3 and its cost 50 to 100 $?

scientific journals for publish with impact factor between 2.5 to 3 and its cost 50 to 100 $

08 September 2015 8,682 0 View

Is anyone working on an emeA efflux pump in enterococci?

Can it work on whole DNA or plasmid only? What is its condition to work on PCR ?

10 November 2014 9,328 2 View

How can I convert PM into ul ?

PM=?ul

03 April 2014 3,255 2 View

How can i prepare master mix for a PCR reaction?

03 April 2014 8,529 21 View

How can I dilute the master mix for use?

03 April 2014 1,226 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I need JCPDS file of LSFCO nanomaterial. Can anyone provide me?

Require for Rietveld refinement in XRD.

08 August 2024 3,081 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View