Is anyone working on an emeA efflux pump in enterococci?

More Aalaa Samir's questions See All

What is the equation of detection n= of DNA copies in conventional PCR product?

IS there is equation for detection of n= of DNA copies in conventional PCR product by measuring pcr product against spectrophotometer

31 December 2017 7,838 3 View

Can i perform 2 steps real time pcr run on one step?

to perform RT PCR to RNA sample the used technique 1st convert RNA to DNA in separate reaction then perform replication can i make them in one step?

11 December 2017 4,630 3 View

Can real time pcr work as conventional pcr?

we have a real time pcr applied biosystems can we use it as thermocycler only or cant ?

05 June 2017 9,871 3 View

How to examine lecithinase activity of Enterococcus spp. on egg yolk agar ?

i prepared egg yolk agar 3%, 1% egg yolk (v/v) on BH agar but cant have result how can prepare it?

04 May 2017 6,647 4 View

I used arginine hydrolysis broth but didn t give any positive result can anyone explain this?

I used this broth according to manufacture recommendation in preparing the media http://www.titanmedia.in/images/upload/td/TM%201140_ARGININEDIHYDROLASEBROTHTM-1140.pdf

08 September 2016 6,682 4 View

How can I extract DNA by Tris–EDTA buffer and Triton X 100?

how can i make stock solution from both solution for direct use & how much mix solution use on each isolate ?

10 November 2015 9,594 3 View

What are scientific journals for publish with impact factor between 2.5 to 3 and its cost 50 to 100 $?

scientific journals for publish with impact factor between 2.5 to 3 and its cost 50 to 100 $

08 September 2015 8,682 0 View

How can I convert PM into ul ?

PM=?ul

03 April 2014 3,255 2 View

How can i prepare master mix for a PCR reaction?

03 April 2014 8,529 21 View

How can I dilute the master mix for use?

03 April 2014 1,226 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

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28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

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Isolation of PLV6-Bmal-Luc plasmid?

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Christian Paulenz

G'Day,

in my opinion, tho following papers are the right thing for you.

But in general, conditions for PCR always belong to buffers, primers and additives (e.g. DMSO). This answer may not helps at the Point, but i hope it will Show you a inspiring way.

Good Luck

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC90871/#B15

http://jid.oxfordjournals.org/content/178/5/1416.long

Aalaa Samir

thanx