What is the equation of detection n= of DNA copies in conventional PCR product?

IS there is equation for detection of n= of DNA copies in conventional PCR product by measuring pcr product against spectrophotometer

31 December 2017 7,720 3 View

Can i perform 2 steps real time pcr run on one step?

to perform RT PCR to RNA sample the used technique 1st convert RNA to DNA in separate reaction then perform replication can i make them in one step?

11 December 2017 4,516 3 View

Can real time pcr work as conventional pcr?

we have a real time pcr applied biosystems can we use it as thermocycler only or cant ?

05 June 2017 9,749 3 View

How to examine lecithinase activity of Enterococcus spp. on egg yolk agar ?

04 May 2017 6,495 4 View

I used arginine hydrolysis broth but didn t give any positive result can anyone explain this?

08 September 2016 6,526 4 View

No title

10 November 2015 9,475 3 View

What are scientific journals for publish with impact factor between 2.5 to 3 and its cost 50 to 100 $?

scientific journals for publish with impact factor between 2.5 to 3 and its cost 50 to 100 $

08 September 2015 8,565 0 View

Is anyone working on an emeA efflux pump in enterococci?

Can it work on whole DNA or plasmid only? What is its condition to work on PCR ?

10 November 2014 9,215 2 View

How can I convert PM into ul ?

PM=?ul

03 April 2014 3,095 2 View

How can i prepare master mix for a PCR reaction?

How can i prepare master mix for a PCR reaction?

03 April 2014 8,292 21 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View