How can I dilute the master mix for use?

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What is the equation of detection n= of DNA copies in conventional PCR product?

IS there is equation for detection of n= of DNA copies in conventional PCR product by measuring pcr product against spectrophotometer

31 December 2017 7,838 3 View

Can i perform 2 steps real time pcr run on one step?

to perform RT PCR to RNA sample the used technique 1st convert RNA to DNA in separate reaction then perform replication can i make them in one step?

11 December 2017 4,630 3 View

Can real time pcr work as conventional pcr?

we have a real time pcr applied biosystems can we use it as thermocycler only or cant ?

05 June 2017 9,871 3 View

How to examine lecithinase activity of Enterococcus spp. on egg yolk agar ?

i prepared egg yolk agar 3%, 1% egg yolk (v/v) on BH agar but cant have result how can prepare it?

04 May 2017 6,647 4 View

I used arginine hydrolysis broth but didn t give any positive result can anyone explain this?

I used this broth according to manufacture recommendation in preparing the media http://www.titanmedia.in/images/upload/td/TM%201140_ARGININEDIHYDROLASEBROTHTM-1140.pdf

08 September 2016 6,682 4 View

How can I extract DNA by Tris–EDTA buffer and Triton X 100?

how can i make stock solution from both solution for direct use & how much mix solution use on each isolate ?

10 November 2015 9,594 3 View

What are scientific journals for publish with impact factor between 2.5 to 3 and its cost 50 to 100 $?

scientific journals for publish with impact factor between 2.5 to 3 and its cost 50 to 100 $

08 September 2015 8,682 0 View

Is anyone working on an emeA efflux pump in enterococci?

Can it work on whole DNA or plasmid only? What is its condition to work on PCR ?

10 November 2014 9,328 2 View

How can I convert PM into ul ?

PM=?ul

03 April 2014 3,255 2 View

How can i prepare master mix for a PCR reaction?

03 April 2014 8,529 21 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View