commercial ELISA kits for estimation of beta hydroxybutyric acid (BHB) cost approx. Rs.50000/- which is very costly. Is there any alternative method for quantitative determination of BHB in blood/ urine/ milk
Hello Sudhakar Goud Karpurapu
You could refer to the following papers for the quantitative estimation of beta-hydroxybutyrate.
https://onlinelibrary.wiley.com/doi/full/10.1002/%28SICI%291520-7560%28199911/12%2915%3A6%3C412%3A%3AAID-DMRR72%3E3.0.CO%3B2-8
Article Automated kinetic method for D-3-hydroxybutyrate in plasma or serum
Article Optimized Kinetic Method for Automated Determination of β-Hy...
Hope this helps.
Best.
Specimen type: β-hydroxybutyrate levels can be measured in serum. Point-of-care devices that can measure β-hydroxybutyrate levels in a single drop of blood within 30 seconds have been developed Sudhakar Goud Karpurapu
Do you have access to a gas chromatography system? Esterification or silylation will render it volatile.
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