Please suggest a non-red and non-green fluorescent bacterial viability stain?

More Sudhakar Pagal's questions See All

What are the factor in prioritization of school heads on programs and projects?

Best Prioritization Practices of school heads on programs and projects

30 January 2024 6,095 4 View

What could be the maximum length of an endoscope to view arodent in a hole?

This device would help to detect enemy or their `store of arms in tonels.

24 October 2023 3,656 0 View

How many fresh-hatched eggs of the Palawan Forest Turtle are required to obtain a sufficient amount of mitochondrial DNA for DNA barcoding?

I will use the Oxford Nanopore MinIon. I will just access the COI gene region.

13 October 2023 6,697 1 View

Could an endoscope view objects in mile long distantce in underground tunnels?

An endosope is used to view sites in side tubal structures of our body like somach, intestine trachea etc. Which is about 9 meters long. Could it be used to view longer distance like killomiters...

01 October 2023 7,955 2 View

How to calculate real and imaginary part impedance from the dielectric ???

I try to plot cole-cole plot so could give some information how to calculate real and imaginary impedance.

07 September 2023 773 4 View

What is the difference between metal ions and rare earth ions ???

both are gives same optical properties or not ?

27 July 2023 7,339 6 View

Egg or hen, which came first ?

It is a perplexing question; everybody wants to know. Scientific knowledge on creation of everything, rather most of the things, is but quite recent. Life started first as living cells. They had...

20 June 2023 2,391 0 View

What are the Global Warming Potentials of various criterion air pollutant such as Carbon Monoxide (CO), Ozone (O3) and Nitrogen Dioxide (NO2) ?

I am working on a paper regarding the impact of air pollution on warming the Global Climate. So, I need to know about the Global Warming potential values of different air pollutants. Therefore, I...

12 May 2023 6,352 2 View

What are the pesticides used in the regular fruits and vegetable that cannot be detected and felt by normal eye sight?

I am seeking information of list of pesticides and drugs that are used to ripe a fruit or change the size or also kind of

12 April 2023 150 0 View

Is Multiplicative interaction necessary and sufficient condition for multifractal scaling?

Hi, Repeatedly, I am coming across literature in multifractal scaling where they reach the conclusion that observation of multifractal phenomena could be due to multiplicative interaction...

16 June 2022 9,180 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Arvind Kumar Shukla

Certainly! One non-red and non-green fluorescent bacterial viability stain that you can consider is SYTOX™ Blue.

SYTOX™ Blue is a high-affinity nucleic acid stain that is impermeable to live bacterial cells but can penetrate membranes of dead or compromised cells. It binds to DNA with high affinity and emits blue fluorescence upon binding, allowing for the differentiation between live and dead bacterial cells.

Here are some key features of SYTOX™ Blue:

Fluorescence: SYTOX™ Blue emits blue fluorescence when bound to DNA, making it easily distinguishable from green and red fluorophores commonly used in fluorescence microscopy and flow cytometry.

Viability Assay: It is commonly used as a viability stain in bacterial cell culture studies, allowing researchers to assess the viability of bacterial populations in a sample.

Versatility: SYTOX™ Blue can be used in various applications, including fluorescence microscopy, flow cytometry, and high-throughput screening assays.

Compatibility: It is compatible with a wide range of bacterial species and can be used in combination with other fluorescent dyes or stains for multiplexing experiments.

When using SYTOX™ Blue or any other viability stain, it's essential to optimize staining conditions such as concentration, incubation time, and buffer composition to achieve the best results for your specific experimental setup and bacterial strains. Additionally, always follow the manufacturer's instructions and recommended protocols for staining and imaging procedures.

Aaron Dhanda

DAPI will stain them and make them visible by fluorescence microscopy

Boon Kuan Woo

May consider using Permai fluorescence dye.