How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

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How can I know the expression level of common tumor targeting receptors on cancer cell lines? For example the cell lines and receptors I mentioned in the question. Thanks a lot.

31 August 2020 5,347 3 View

How can I get H-bond versus time graph and hydrophobic residue distance versus time graph from Gromacs ?

I am using Gromacs to analyze the interactions between protein-ligand.I am a beginner of Gromacs simulation. I have finished the simulation, I got the final gro , xtc, cpt, edr files, but not...

05 August 2020 3,401 3 View

How to make parrallel inlet boundary UDF work?

Hi everyone, It is quite urgent for me! I am doing 2D rotating detonation engine (RDE) simulation in ANSYS Fluent. For inlet boundary condition, there are three possible cases: 1. Pi > Po,...

02 August 2020 8,443 1 View

Preparation of PVDF Piezoelectric Sensor?

My main research is electrospinning of PVDF as piezoelectric sensors. According to the description in papers, I "Used PI/PET film as the substrate, pasted conductive tape as one side electrode,...

19 July 2020 5,287 2 View

2D rotating detonation simulation - How to implement ANSYS UDF boundary condition?

Hi everyone, I am doing 2D rotating detonation engine (RDE) simulation in ANSYS Fluent. For pressure inlet boundary condition, there are three possible cases: 1. Pi > Po, where Pi is the...

25 May 2020 7,040 16 View

How to solve CLUE-S model error "error in file sc1gr0.fil"?

I don't know where the problem is since I have checked the format of thoes files. I wish somebody can help me with this problem. Thanks!

13 May 2020 5,474 1 View

The collagen gel evaporate with 3D organoid model (air Liquid interface method), why?

Hi, guys, I have a queation, please help me. I used air liquid interface method to do 3D culture. This is the first time to do it. the collagen gel prepared according to literature, 8 vol of...

13 May 2020 7,288 2 View

Why OCP is differnet when test the same BFC with fruits and animals?

I developed a BFC (GOD anode and BOD cathode), tested it with apple and mouse's blood, the OCP with apple was 0.62V, but the OCP with mouse's blood was only 0.5V, I thought the OCP should be same...

25 March 2020 2,866 1 View

How do we judge the mediation hypothesis was supported or not if BC interval confidence did not include zero, but estimate is small?

Hi, I'm a postgraudate student and thanks all in advance for kindly giving me some suggestions and comments. I'm testing a model with multiple independent variables and mediators using...

17 December 2019 8,994 6 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View