Hi, currently I am isolating miRNA from hematopoietic stem cell using miRNeasy Qiagen kit. However, I didnt get good purity for both A260/280 and A260/230 plus low concentration as well. According to article, entittled " Aberrant miRNA profiles associated with chronic benzene poisoning" written by Bai et al. 2014 from from ELSEVIER journal (Experimental and Molecular Pathology) , they have extracted the total RNA using both TRIZOL and miRNeasy Mini Kit (Qiagen). I am not sure about the protocol how they modify and use TRIZOL together with the kit. Hope can help me in this.. Thanks.