How to do cryosection of bone marrow from mouse femur?

08 March 2024 0 2K Report

I want to see adipocytes from this material in slides with oil red. I can decalcify and place in OCT by making cuts on silanized slides? How thick is the cut? Can my cryostat cut decalcified material (Leica cm1850)? How long do I leave it in EDTA?

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