Dear all,
I patch dissociated neuronal cultures and would like to have more precise overview of neuronal types. I can distinguish neurons while patching them but it would be more helpful to know approximately which type I am going to patch while I am looking for one.
Do you know any staining (not harmful) to differentiate types and not to interfere with the physiological properties of neurons?
Or at least to tell if it is excitatory or inhibitory neuron?
Thank you a lot in advance!
Hy,
you could work with transgenic animals expressing GFP under the promoter of a specific neurotransmitter synthetising enzyme:
- For catecholaminergic neurons tyrosine hydroxylase
- For GABA I'm sure that there should be GAD67 transgenic animals and so on......
I'm not sure that immunohistechistry would work on living cells, because most neuronal markers are intracellular epitopes.
Kind regards
Stefan
You can distinguish interneurons by the morphology. You may want to try staining fixed neurons with CTIP2 for CA1 and DG neurons once and see if you can find any morphological signatures usable for patching. I think there is a marker for CA3 neurons but cannot remember at the moment.
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