Oleksandra Tiapko

4 Questions 3 Answers 0 Followers

Questions related from Oleksandra Tiapko

Can I apply cholesterol dissolved in DMSO to activate ion channels?

I do patch clamp (and Ca2+ imaging) and usually apply DAGs-DMSO in external buffer through the perfusion. Now I want to see if my ion channels will respond to cholesterol. I prepared...

06 August 2020 244 0 View

Special tricks for current clamp recording of cerebellar dissociated neurons?

Hi guys, I want to patch dissociated cerebellar neurons. I was successfully growing them for 7 days already and now want to do current clamp measurments. However, when I obtain whole cell I cannot...

22 August 2016 1,318 4 View

Has anyone had a problem with HEK293 insensitivity to Carbachol?

Hi everybody! Would be very thankful if someone can help with a small problem! Suddenly HEK293 became insensitive to Carbachol stimulation in FURA experiments. With application of Carbachol no...

24 November 2015 2,101 3 View

How to distinguish neuronal types in dissociated hippocampal culture

Dear all, I patch dissociated neuronal cultures and would like to have more precise overview of neuronal types. I can distinguish neurons while patching them but it would be more helpful to know...

01 January 1970 3,514 2 View