How to dissolve xylan (1%) for xylanase assay. It is not soluble in water. would increase in temperature to 100oC dissolve xylan??

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Mukund G Adsul

prepare 1% solution of xylan in suitable buffer. keep this mixture in Boiling waterbath for 20 min. approx. 50% xylan solubalized. use this directly for xylanase assay

Khelifa Bouacem

Dear Mamatha Pingili,

You can see this article: Partial Characterization of Xylanase Produced by Caldicoprobacter algeriensis, a New Thermophilic Anaerobic Bacterium Isolated from an Algerian Hot Spring (Bouacem et al., 2014);

Best regards

Mamatha Pingili

Thank you mukund G Adsul for your advice. Will try it

Thank you Klelifa Bouacem for your response . Will go through the article.

Vladimir Elisashvili

Please see the attached paper

Thank you Vladimir Elisashvili for your response

Tamer YA Fahmy

The below mentioned researcher has many articles on hemicelluloses (including xylan). Happy new year.

https://www.researchgate.net/profile/Yehia_Fahmy