Hello.. did any of you ever do a native PAGE and SDS PAGE for xylanase enzyme production from Trichoderma sp.. I need a procedure plz
Check this for recipes
Although optimized for DNA/ RNA, can be used for proteins too, same chemistry
Article A Comprehensive Analysis of Northern versus Liquid Hybridiza...
Hi Rana,
You can take a look at this article where we performed SDS-PAGE and Immunoblotting with fungal enzymes including xylanase from Trichoderma [Int Arch Allergy Immunol 2007;144:231-9. PMID: 17587859].
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