Good day! Recently we have obtained binary composites of cellulose with hemicellulose additives (xyloglucan, xylan, arabinoxylan and glucomannan). We want to establish an experiment, dedicated to enzymatic treatment of material in order to remove hemicelluloses from its matrix. Specific hemicellulases will be used:
- xyloglucan-specific endo-beta-1,4-glucanase (EC 3.2.1.151; endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan);
- endo-1,4- β-D-xylanase (EC 3.2.1.8; hydrolysis of xylans to xylan oligosaccharides);
- endo-1,4- β-D-xylanase (EC 3.2.1.8; hydrolysis of xylans to xylan oligosaccharides) + α-L-arabinofuranosidase (EC 3.2.1.55; hydrolysis of α-1,2- and α-1,3-linked L-arabinofuranose residues from arabinoxylans);
- endo-1,4- β-D mannanase (EC 3.2.1.78; hydrolysis of beta-1,4-D-mannosidic linkages in glucomannans).
However, we have lack of expertise on working with enzyme.
So, the question is are there any specific protocols or requirements for an enzymatic treatment of hemicelluloses?
What is the temperature of the process, pH, what quantity of hemicelulases should we use?
What should be the duration of the process?
We are trying to set up something like in this article:
Article Mechanical effects of plant cell wall enzymes on cellulose/x...
Many thanks for sharing your thoughts and ideas!
There are a few general guidelines to follow when performing an enzymatic treatment of hemicelluloses:
It is also important to note that hemicellulases are generally more sensitive to environmental conditions such as temperature and pH compared to other enzymes. Therefore, it is important to carefully control these variables to ensure optimal enzyme activity and avoid denaturation.
Good day, Suganth Velumani ! Thank you for your kind answer. We have already planned that experiment. Hope we will do it in several month and report our results in 2023.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Materials
- Inorganic Materials
- Metals
- Alloys
- Steel