i dissolved in PBS ph 7.2 and also in kcl buffer ph 7, 0.5% triton also added. Then I did dialysis for 42 hrs. but some white remain and setted down . Kindly any tell me how to dissolve lipase ?
i want to askl the journal in the feild of ayurdedic or herbal drugs with good impact factor?
10 November 2018 2,127 1 View
I am using lipase of aspergillus niger for lipase inhibiton assay against some molecules. using substrate olive oil. Kindly if anybody have protocol? Thanks
01 February 2018 8,626 3 View
I am using b GLUCANASE of Aspergillus niger for my exxperiments. If anyone can suggest in which buffer anf pH should I dissolve this enzyme. Kindly guide me the possible way. Thanks
01 February 2018 1,231 6 View
Can anyone guide me for the ph and concentration. I have used 4 to 1uM of concentration in phosphate buffer at pH 7.4 and 7.5 too. I have excited at 290 and getting emmision at 334. But no...
11 December 2017 8,289 3 View
I purchased lipase. It is not soluble in water. After doing dialysis, scattering and aggregation has been noticed in UV spectra . What should I do to prevent the above same?
09 October 2017 4,992 3 View
(protein + ligand interactions)
07 August 2017 9,661 2 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...
06 August 2024 1,350 4 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View