Can anyone tell the journal in the feild of ayurdedic or herbal drugs with good impact factor?

i want to askl the journal in the feild of ayurdedic or herbal drugs with good impact factor?

10 November 2018 2,029 1 View

Can anybody provide protocol for the lipase inhibition assay using olive oil ?

I am using lipase of aspergillus niger for lipase inhibiton assay against some molecules. using substrate olive oil. Kindly if anybody have protocol? Thanks

01 February 2018 8,540 3 View

Solubility of b GLUCANASE of Aspergillus niger ?

I am using b GLUCANASE of Aspergillus niger for my exxperiments. If anyone can suggest in which buffer anf pH should I dissolve this enzyme. Kindly guide me the possible way. Thanks

01 February 2018 1,138 6 View

Best suitable pH and concentration for working the binding studies with lipase?

Can anyone guide me for the ph and concentration. I have used 4 to 1uM of concentration in phosphate buffer at pH 7.4 and 7.5 too. I have excited at 290 and getting emmision at 334. But no...

11 December 2017 8,199 3 View

After dialysis of lipase , how to prevent scattering and aggregation for uv absorption spectra.Kindly suggest what to do ?

I purchased lipase. It is not soluble in water. After doing dialysis, scattering and aggregation has been noticed in UV spectra . What should I do to prevent the above same?

09 October 2017 4,896 3 View

Can anybody tell journals for biophysical workabove impact factor 5 ?

(protein + ligand interactions)

07 August 2017 9,577 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View