Hi all,
I am planning to conduct oxidative stress study, and I wonder is there any protocol that can succesfully induce oxidation on the cancer cell line (HTC116) without reduce its viability?
Given that HCT116 has p53 different from many other cancer cell lines, treatment with hydrogen peroxide (H2O2) at the concentration of 50-100 µM for 6-18 hours is appropriate for induction of oxidative stress. From my experience, H2O2 would lose activity after 24 hours in the medium with serum. Importantly, oxidative stress is a double-edged sword; H2O2 treatment at the low concentration activates Akt and Wnt/beta-catenin survival and proliferation signals, whereas high concentration of H2O2 would trigger cellular toxicity. Furthermore, BSO treatment inhibits the synthesis of glutathione (GSH), a major ROS scavenger, thereby, inducing the ROS accumulation. Moreover,sulfasalazine (SSZ) blocks xCT cystine/glutamate antiporter, so that SSZ treatment also reduces the amount of GSH and increases ROS level.
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