As tittle show, is someone know what is the most advance technologies/method in assessing type-II diabetes markers in human tissue?
Hi Scholars, I am planning to conduct an mice study to evaluate the insulin resistance of the mice. In the study, the pancreas will be harvested and the DNA will be purified for the qRT-PCR. Can...
05 June 2016 1,046 8 View
Dear scholars, I would like to isolate both spleen and pancreas from mouse. But I found out that it is hard to conduct pancreatectomy without injure the spleen. Can anyone give some...
03 April 2016 9,414 5 View
Hi scholars, I am planning to conduct western blot to detect MUC2 protein , which estimate have about 540kDA size. Judging by this huge size, I wonder can the protein be electrophoresised and...
03 April 2016 5,708 7 View
Hi all, I am planning to conduct oxidative stress study, and I wonder is there any protocol that can succesfully induce oxidation on the cancer cell line (HTC116) without reduce its viability?
03 April 2016 5,671 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View