1. First, after the passage of the Thp1, the cells prefer to grow aggregately, not grow in single-cell, which means the density is high?

2. Second, I use 100nM PMA to differentiate Thp1 for 48h, then use the PMA-free medium to rest the cells for a week. The morphology is the same as in the other articles, but after the passage, the morphology becomes stretched and back to circle suspension status.

3. So M0 cannot sustain so long and I need to incubate with other reagents to M1/M2 status?