Dear All
I already found the result for phenolic compound that analyzed by UHPLC-ESI-QTRAP-MSMS as file attached. My problem is how to differentiate between phenolic acid and flavanoid? From these results, it can be seen that the analysis of the pitaya peel extract with UHPLC able to identify two types of phenolic acids which are hydroxybenzoic acids and hydroxycinnamic acids. The result showed that, hydroxycinnamic acids represent Quinic and Cinnamic acid and hydroxybenzoic acids represent protocatechuic Hexoside conjugate . How about the rest of compound identified? I just know the peak eluting at 1.42 min displayed the deprotonated molecule at m/z 191.40, which fragment to the ion of m/z 172.92 (-18 Da; neutral loss of water, allowed to identify it as quinic acid. Further product ions at m/z 110.98 reinforced this structure proposal (bastos et al, 2007).
Regards
Azlan
hi
you can see my article ..i hope its useful
Article Effect of Aqueous and Alcoholic Plant Extracts on Inhibition...
Hi sir olusegen. I don't have targeted compound. I just use MSMS for confirmation identified compound in my salution extraction. But i don't know how to explain all these compounds.Their name is quite strange for me
Hello Azlan, I identified these same compounds in my article using the same technique, I hope it can help you.
If you don't have any targeted compounds in mind. I will suggest that you carry out both positive and negative ions mode using LCMS-MS, because phenolic compounds are in negative mode while flavonoid compounds are in positive mode.
Other than this, you can as well determine the quantities of total phenolic content and total flavonoid content in your extract. I believe these attached articles will help.
hi lasnaia. Thanks for sharing article. May I know do you use qtrap or qtof to determine phenolic compound in your sample?
For identification, an Ion Trap ESI-MS/MS detector was used.
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