I can't synthesize standards for BM adducts since they need a toxic reducing agent (NaBH4).
To my knowledge (forgive the undergrad me), without the standards, I wouldn't be able to know what the retention time of the adducts are. (There may be literatures stating their RT but the HPLC conditions may differ.)
Now, how would I determine that certain peaks on the chromatogram of the extracts correspond to the BM adducts (extension units) without the standards' RTs? Henceforth, calculate mDP.
Can someone clarify these things?
The retention times are very different while the spectra are very similar (btw, I have analysed procyanidins for many years and i do not understand why the NaBH4. (or why you are scared of it, we use it routinely for sugar reduction in alditol acetates, a hood will work and it is less a problem than benzylmercaptan).
Read the articles by S. Guyot I recommended last time, they are quite clear on the methodology. Or invest in some procyanidin B2 and run the chromatogram.
Mrs/Miss Rado,
Please, consider the content of the following discussion:
[https://www.researchgate.net/post/How_to_seperate_two_components_by_RP-HPLC_having_very_near_pKa_values_ie_1298_and_1338].
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