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Questions related from Reena Dado
Regarding the highlighted part of the photo below, is the blank really the one with no catechin? How can it be so since catechin standard is added to all the test tubes (except for the 0mL...
21 September 2021 7,228 3 View
This is the method that I saw in a paper we are using as basis: low MW tannins were eluted with acetone/water (3:7; v/ v; Fraction 1) and higher MW tannins with acetone/ water (1:1; v/v; Fraction...
11 September 2021 2,281 3 View
Can I use GC-MS instead of LC-MS for confirmation of synthesized benzyl mercaptan adducts (catechin-BM, Epicatechin-BM, Gallocatechin-BM, and Epigallocatechin-BM)? I can't seem to find methods...
14 August 2021 8,216 6 View
I can't synthesize standards for BM adducts since they need a toxic reducing agent (NaBH4). To my knowledge (forgive the undergrad me), without the standards, I wouldn't be able to know what the...
09 August 2021 9,672 4 View
I found this video (silica gel was used): https://www.youtube.com/watch?v=8Ff1Xx-On-g&t=96s Can I use this process to pack my sephadex onto the column? (yes, right after I swell sephadex).
04 August 2021 4,131 6 View
I am just an undergraduate student and the reagents we need are really expensive in sigma-aldrich. I had found a company that offers them in a more affordable price and lower MOQ (name is...
03 August 2021 2,501 10 View
Sephadex LH-20 is too expensive. We have silica gel, but I can't find methods on how to fractionate the tannin extract. We are specifically interested in the condensed tannins. Can someone please...
07 June 2021 377 8 View
The manual for Sephadex LH-20 says this: "Resuspend and pour the media slurry into the column in one continuous motion. Fill the reservoir to the top with buffer." However, I saw another...
07 May 2021 9,589 1 View
We are extracting tannin from plant. The method says that after treating the plant sample with acetone/water (7:3; v/v), solutions are then concentrated, then the aqueous extracts were...
04 May 2021 7,040 10 View
what instruments are needed to determine and quantify: PD:PC ratio mean degree of polymerization cis/trans stereochemistry? As much as possible, the simplest instrument is preferred. This is for...
02 May 2021 9,745 2 View
If yes, can you pls provide a methodology for this? How about if L3 is straight from an animal source? Will this be much easier? How would I know if the animal has monospecific infection? Pls...
28 April 2021 8,323 2 View
