gene expression profile test

Hi Helen, 

First, you could perform a dot/slot blot and stain your DNA with antibodies against N7meG and O6meG. The latter is regularly performed in our lab (https://academic.oup.com/carcin/article/36/10/1235/316766/DNA-repair-by-MGMT-but-not-AAG-causes-a-threshold ; Fig 3B). 

The second method is to perform a HPLC mass spec analysis with isotopically labeled spike-in standards (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4090024/). But that's really expensive if don´t have it in house.

good luck, olli

You can use our recently published paper for genome-wide mapping of N7meG and N3meA, but not O6meG. Link: https://genome.cshlp.org/content/early/2017/09/14/gr.225771.117.abstract

Hope it is helpful for you!