What solvent is best for dilution of MTBSTFA+1%TBDMS derivatives of chemicals?

What solvent(s) would experts recommend to dilute the MTBSTFA+1%TBDMS derivatives of polar compounds prior to GC/MS injection.

09 October 2018 8,655 0 View

Difference in trypsinization and scrapping to harvest cell for intracellular metabolites?

I would like to know if there is any difference in harvesting mammalian cells via trypsinization or scrapping for intracellular metabolite analyses. The metabolites could be polar no non-polar...

08 September 2018 5,715 0 View

How many Journals are there which publishes concept paper not research articles?

I am looking for journals related to Biotechnology, Chemistry and Enzyme catalysis but not limited. I will provide further details if necessary.

10 November 2017 8,696 0 View

Is UDP/TDP-beta-D-glucose commercially available?

Is it possible to buy UDP/TDP-beta-D-glucose from any commercial source with reasonable price?

07 August 2016 520 5 View

Does single glycosyltransferase has inversion as well as retention property?

01 January 1970 1,717 4 View

What is the chance of UDP-beta-D-glucose formation?

01 January 1970 168 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View