What solvent(s) would experts recommend to dilute the MTBSTFA+1%TBDMS derivatives of polar compounds prior to GC/MS injection.
I would like to know if there is any difference in harvesting mammalian cells via trypsinization or scrapping for intracellular metabolite analyses. The metabolites could be polar no non-polar...
08 September 2018 5,781 0 View
I need to analyze the phosphate containing intermediates like phosphoenolpyruvate, Glyceraldehyde 3-phosphate, 3-phosphoglycerate, etc. from the cellular sample. What is the best analytical tool...
07 August 2018 1,617 0 View
I am looking for journals related to Biotechnology, Chemistry and Enzyme catalysis but not limited. I will provide further details if necessary.
10 November 2017 8,776 0 View
Is it possible to buy UDP/TDP-beta-D-glucose from any commercial source with reasonable price?
07 August 2016 585 5 View
Is it possible a single glycosyltransefrase can have inversion as well as retention properties with a single acceptor substrate? Are there any published references?
01 January 1970 1,792 4 View
What is the chance of UDP-alpha-D-glucose and UDP-beta-D-glucose formation in One-Pot in vitro reaction catalyze by series of enzymes from beta-D-glucose as primary precursor. Is there any chance...
01 January 1970 228 5 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View