Difference in trypsinization and scrapping to harvest cell for intracellular metabolites?

I would like to know if there is any difference in harvesting mammalian cells via trypsinization or scrapping for intracellular metabolite analyses. The metabolites could be polar no non-polar...

08 September 2018 5,715 0 View

How to detect and quantify phosphate containing metabolites from cell culture?

I need to analyze the phosphate containing intermediates like phosphoenolpyruvate, Glyceraldehyde 3-phosphate, 3-phosphoglycerate, etc. from the cellular sample. What is the best analytical tool...

07 August 2018 1,552 0 View

How many Journals are there which publishes concept paper not research articles?

I am looking for journals related to Biotechnology, Chemistry and Enzyme catalysis but not limited. I will provide further details if necessary.

10 November 2017 8,696 0 View

Is UDP/TDP-beta-D-glucose commercially available?

Is it possible to buy UDP/TDP-beta-D-glucose from any commercial source with reasonable price?

07 August 2016 520 5 View

Does single glycosyltransferase has inversion as well as retention property?

01 January 1970 1,717 4 View

What is the chance of UDP-beta-D-glucose formation?

01 January 1970 168 5 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View