I want to detect tunicamycin inside extracellular vesicles and cells. Does anyone know how to do this?
How can I analyse the relationship between five independent variables and one dependent variable, with a moderating variable influencing each of these five relationships? How can I test hypotheses...
03 June 2024 6,221 1 View
We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...
28 May 2024 3,529 1 View
We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....
28 May 2024 6,052 2 View
Hello. I am here looking for help with the NO radical scavenging assay. I was working on setting up the assay with sodium nitroprusside and the Griess reagent as they do in most works. However, I...
27 May 2024 5,013 1 View
Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...
21 May 2024 2,171 0 View
Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.
16 May 2024 9,432 1 View
I want to evaluate the polarization to M2 phenotype in Raw Cells, I co-cultured the cell with LPS and my drug to see the effect of the drug to inhibit the proinflammatory effect of LPS, but I have...
05 May 2024 8,525 0 View
Sirs. ResearchGate Receive a cordial and respectful greeting. For academic purposes only, we request collaboratively the following articles for one of our research professors, who is preparing...
05 May 2024 9,459 9 View
How can we prevent small peptides (1000 Da) from eluting out of the gel during staining in a peptide gel (tricine-SDS-PAGE, Shagger)? There are different types of fixing solutions, but I'm not...
28 April 2024 1,522 0 View
I am currently conducting research for my PhD on environmental regulations, specifically focusing on the disposal of produced water from oil and gas activities in Australia. I would appreciate any...
18 April 2024 1,544 1 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...
15 July 2024 4,192 1 View
Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...
13 July 2024 9,783 1 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
In my country, there is a lot of public outcry in relation to the presence of drug injection and related behaviors in public. Hence at least the bigger cities would probably benefit from the...
08 July 2024 8,903 1 View
Hello everyone reading this topic! It is well known that microcrystalline cellulose 102 is recommended for use in direct compression technology, however, like other grades, it has poor flowability...
08 July 2024 2,807 0 View