Ana Sayuri Yamagata

5 Questions 11 Answers 0 Followers

Questions related from Ana Sayuri Yamagata

What's wrong with my HUVEC in culture?

I have been culturing HUVEC and sometimes the cells look stressed and stop dividing. When this happens, they don't recover after I change the medium. What may be causing this problem? Expired...

24 September 2023 2,552 3 View

How to detect a drug inside extracellular vesicles and cells?

I want to detect tunicamycin inside extracellular vesicles and cells. Does anyone know how to do this?

30 November 2022 1,665 0 View

How many times can a treated culture plate be reused after wash and iradiation?

We have been washing treated culture dishes with hipoclorite and extran and iradiating to use them again. How many times can we do that without detaching or stressing the cells? Do you have any...

10 October 2021 4,276 2 View

What is the best way to detect ATF4 activation? And ATF6?

To detect by WB, I would have to isolate the nucleus fraction, right? But I did whole protein isolation. Has anyone detected ATF4 or ATF6 by histology? Thank you

18 September 2020 3,170 1 View

Is it possible to isolate exosomes released from non-adherent adipocytes?

I fear that exosomes would not be in the medium, but in the same density as adipocytes.

04 February 2019 8,982 5 View