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Questions related from Ana Sayuri Yamagata
I have been culturing HUVEC and sometimes the cells look stressed and stop dividing. When this happens, they don't recover after I change the medium. What may be causing this problem? Expired...
24 September 2023 2,547 3 View
I want to detect tunicamycin inside extracellular vesicles and cells. Does anyone know how to do this?
30 November 2022 1,661 0 View
We have been washing treated culture dishes with hipoclorite and extran and iradiating to use them again. How many times can we do that without detaching or stressing the cells? Do you have any...
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To detect by WB, I would have to isolate the nucleus fraction, right? But I did whole protein isolation. Has anyone detected ATF4 or ATF6 by histology? Thank you
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I fear that exosomes would not be in the medium, but in the same density as adipocytes.
04 February 2019 8,977 5 View