Ali Malik

3 Questions 2 Answers 0 Followers

Questions related from Ali Malik

How to fix the issue of pelleting of soluble recombinant protein during purification?

I am working with a DNA binding protein 180 Kda. after Heparin Purification or Gel filtration, when i used to centrifuge the purified protein at 10,000 xg, 10 mint, pellet is observed sometimes...

20 March 2024 6,017 4 View

How to design vector for overexpression of a heterodimer protein ?

human Topoisomerase is a HOMODIMER Protein (AA). Now i want to mutate one chain to produce a heterodimer version (AA*). but there are chances of production of three products (AA, AA*, A*A*). So...

05 December 2023 8,827 1 View

In protein purification, A260/A280 = 0.6-0.8 is ideal. >0.8 shows DNA contamination. What is indicated by <0.5? is it also show contamination?

Theoretically, if the value of A260/A280 goes high it shows DNA contamination in protein sample, and if goes down (0.6-0.8) it shows good purity of protein. In this way, a value less than 0.6...

17 April 2023 7,856 3 View