In protein purification, A260/A280 = 0.6-0.8 is ideal. >0.8 shows DNA contamination. What is indicated by <0.5? is it also show contamination?

Theoretically, if the value of A260/A280 goes high it shows DNA contamination in protein sample, and if goes down (0.6-0.8) it shows good purity of protein. In this way, a value less than 0.6...

17 April 2023 7,846 3 View