How to design in-silico oligonucleotide or small interfering RNA Molecule for particular Gene silencing ?

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Any leads on how Frequency Selective Scheduling is achieved in 5G Network?

Hi everyone, Does anyone know how Frequency Selective Scheduling is implemented in a 5G Network? A step-by-step understanding would be appreciated. Any references or articles will be good. Thanks.

30 July 2024 4,963 2 View

Is there any difference or enhancement in the usage/implementation of Proportional Fairness Scheduling in LTE(4G) and NR(5G)? Any articles or leads?

Any leads with proper explanation would be helpful.

02 July 2024 5,649 2 View

Can specific capacitance increases with increase in current density?

In my case , as i increase the current density the specific capacitance of the electrode is increasing. Also, I am getting the least capacitance in case of 1 A/g and maximum at 10 A/g. Can anyone...

19 May 2024 1,422 0 View

To isolate gingivaMSC,epithelial & fibroblast from biopsy tissue. Which protocol is best for the isolation process?what media is best for the same?

I have been trying various protocols, I was successful at isolating cells from tissue via both explant and enzymatic method, however after some days the cells are becoming stagnant and are not...

14 May 2024 8,846 0 View

How to do lncRNA annotation in R after making count matrix?

Can anyone please

30 April 2024 1,444 0 View

How to quantify the fluorescence intensity of a confocal image with Fiji (ImageJ)?

I have taken fluorescence images of the control and treated sample(Immunofluorescence, tissue sample) at the same settings. So I need to measure the change in fluorescence intensity of the treated...

16 April 2024 2,093 1 View

How much execution time is normal for completion of a tensorflow dataset generation function when working on a GPU accelerated workstation?

I am working on a deep learning model with following GAN(Generative Adversarial Network) architecture and got stucked on dataset creation(using tensorflow function to simplify the data for my...

05 April 2024 6,628 1 View

How to calculate and select the treatment dose for combination of two drugs ?

currently, i am working on cancer cells and trying to study the effects in combination. One is a known anticancer drug but the other does not show any anticancer properties however it can reduce...

17 March 2024 9,764 1 View

Not getting fiber deposition for electrospinning of Poly lactic acid (chloroform/DMF solvent) on collector. what can be the possible reason?

Hello everyone! I am preparing electrospun fiber of PLA in Chloroform/DMF solvent (9:1 ratio)...I am following standard reported protocol but i am not getting fiber deposition on collector much....

15 March 2024 4,608 2 View

What areas can be explored for undergraduate thesis research on Deep Learning on EEG?

I am inclined to research on EEG classification using ML/DL. The research area seems saturated. Hence, I am confused as to where I can contribute.

14 March 2024 7,060 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Oscar L. Sierra

https://www.researchgate.net/post/How_to_design_a_siRNA

There are other useful links as well within researchgate.

Lukasz Kuryk

Hi, this paper might help: Article Computational design of small interfering RNAs and small hai...

https://www.invivogen.com/sirnawizard/guidelines.php

Dipjyoti Dey

Thank you