How to design an mmu-mir-34a MSP primer or who already has it?
Hi, I'm trying to study the impact of reactive oxygen species on whole blood with respect to how it may induce proteolysis on an intracellular protein in PBMC's in a simulated extracorporeal...
10 October 2021 1,763 7 View
Dear SPM/fMRI users, I am currently running Finite Impulse Response (FIR) analyses on SPM12. Despite everything going well in the classic HRF analysis, now SPM output some error message for...
15 September 2020 5,460 1 View
Been working with myeloma cell lines, primary myeloma cells and PBMCs. Any efficient and feasible methods to measure nuclear pH?? Thanks in advance!
25 August 2019 4,296 3 View
Dear all I am currently conducting an experiment that would require whole-brain EEG, eye-tracking system, and biofeedback signals recording. Besides, participants in this experiment would be asked...
08 August 2018 6,206 5 View
Hi all, I have a two-level network. Nodes in each level have its own edges, and there are connections between levels. Also, the weight of nodes in the first level are different. How could I...
05 June 2018 8,043 0 View
I'm trying to do a project using western blot to assay the level of a cell surface protein that is currently without established norm in pediatric or adult populations. In order to do the assay, I...
21 December 2017 4,989 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
