Been working with myeloma cell lines, primary myeloma cells and PBMCs. Any efficient and feasible methods to measure nuclear pH??
Thanks in advance!
You can refer - Journal of Cell Science 109, 257-262 (1996)
The best biological pH detector is SNARF-1. Read the attached file.
Thanks for your answers Mrinalini Sharma and Alireza Naderi Sohi .
Hi, Recently I was running VASP to calculate the spontaneous polarization of my alloy structure. I followed the instructions in this PDF wrote by Dr. Nabin (Nabaraj) Pokhrel, below is my INCAR...
07 August 2024 486 5 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...
27 July 2024 8,508 0 View
I am a beginner with ImageJ and would like to know how to quantify fluorescence images of platelets taken with a fluorescence microscope at 40x and 100x magnification. If you could provide...
11 July 2024 1,464 7 View
I measured the fatty acids in the fish, using the internal standard method in the national standard method, I first extracted the fish oil with cable extraction, please ask me how many grams of...
02 July 2024 900 1 View
Who could give me an online access to Mandarin Ducks and Butterflies: Popular Fiction in Early Twentieth-Century Chinese Cities by Perry Link?
27 June 2024 1,589 0 View
Hello everyone! I'm conducting a study on how people choose between different shopping channels in today's omnichannel retail environment, and I need your input! Whether you prefer shopping...
26 June 2024 5,275 0 View
Based on my previous question on ReseachGate, I transfected the gene of Norepinephrine Transporter into human colon cells. The sequence of the vector has been confirmed correct. Usually, after...
19 June 2024 1,144 3 View
I wanted to check the expression of a transfected membrane protein called NET, so I decided to check the mRNA by RT-PCR. After three months of culture selection (the cells were still not...
18 June 2024 5,822 3 View
Hi all, Last week, I seeded cells from the same sample into 4 wells (fibroblasts; 300000 cells/well; 1ml/well). well-1 and well-2 are for qRT-PCR test; well-3 and well-4 are for Western blot...
09 June 2024 2,482 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View