We guess that some ssDNA molecules in the nucleus of eukaryotic cells might arrive from DNA to DNA transcription. Besides, the origin of ecDNA is not completely clear, some of which might be formed by ssDNA molecules transcribed from the genome. To prove this, we think of a simple approach in which arbitrarily-primed PCR is used to check samples collected at difffernt points of cell cycle before cell cylce enters S phase. If no extra DNA molecules are produced , the PCR banding patterns will be the same in all samples. However, if extra PCR bands or some bands becoming much bigger are found in some samples, the templates of these extra or bigger PCR bands could be ssDNA molecules transcribed from the genome during cell cycle progression. We ask if this approach is workable? or Is there any other better approach to take?
The results reported in the following paper have suggested that DNA to DNA transcription might exist in eukaryotic cells:
Article Certain Amplified Genomic-DNA Fragments (AGFs) May Be Involv...
You're going to need to clarify your question.
The is no such thing as "DNA to DNA transcription". Transcription is creating RNA from a DNA template.
DNA replication already includes a transient stage with single-stranded DNA in the replication fork. How will you rule out this normal step?
I'm assuming this is some sort of "thought experiment" and not a real research project.
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